The Yorkshireman on Arundel Gate is preparing to call last orders, with its final opening planned for Saturday 10 October.
The Sheffield city-centre pub is marking its closure with a 12-hour farewell featuring DJs, drinks and live music.
The venue briefly closed in January 2024 before reopening three months later under regulars Mark Chambers and Ingrida Kavaliauskaite.
News of the latest closure has prompted messages from customers describing it as the end of an era for another familiar Sheffield venue.
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Source: The Yorkshireman